Experimental and Mathematical Analysis of Regulatory Networks in T-helper Lymphocytes

Edda G. Schulz

ISBN 978-3-8325-2498-2
170 pages, year of publication: 2010
price: 35.50 €
Experimental and Mathematical Analysis of Regulatory Networks in T-helper Lymphocytes
In this book, an interdisciplinary approach combining dynamic quantitative measurements with mathematical modelling is used to solve two different problems in molecular immunology.

In the first part, structure and function of the gene-regulatory network that controls differentiation of type I T-helper (Th1) cells is investigated. By determining the network structure through an iterative process of modelling and experiments, the author shows that Th1 differentiation proceeds in two steps: In the early effector phase, the Th1 master transcription factor T-bet is controlled by an interferon-? dependent positive feedback loop, while in the later phase a second IL-12 dependent feedback maintains T-bet expression. The antigen signal acts as a switch between the two pathways. Moreover, it is shown that only T-bet expression in the late phase is predictive of the success of the differentiation process. Since T-bet expression in the late phase requires IL-12 stimulation, this work uncovers the molecular mechanisms behind the unique role of IL-12 in Th1 differentiation.

In the second part, regulation of the transcription factor NFAT that mediates antigenic stimulation in T-cells is investigated. NFAT is activated by nuclear import upon dephosphorylation of multiple residues. Based on simultaneous measurements of NFAT subcellular localization and phosphorylation, a quantitative mathematical model of the NFAT regulatory network is developed and the underlying design principles are analyzed.

In summary, the study exemplifies the necessity of a dynamic analysis at the systems level to understand complex biological processes.

cover cover cover cover cover cover cover cover cover
  • Systembiologie
  • Immunologie
  • T-Helfer-Zelldifferenzierung
  • Mathematische Modellierung
  • Gen-regulatorische Netzwerke


35.50 €
only 2 in stock

34.50 €
45.50 €
49.50 €

(D) = Within Germany
(W) = Abroad

*You can purchase the eBook (PDF) alone or combined with the printed book (eBundle). In both cases we use the payment service of PayPal for charging you - nevertheless it is not necessary to have a PayPal-account. With purchasing the eBook or eBundle you accept our licence for eBooks.

For multi-user or campus licences (MyLibrary) please fill in the form or write an email to