In a metabolic engineering approach an antibody producing CHO cell line stably expressing human carbonic anhydrase (hCAII), the enzyme that catalyzes the equilibrium of CO2 in aqueous solutions, was generated and used to characterize CO2 effects in simulated CO2 acid load and high CO2 levels as they occur in large scale mammalian cell culture. The cell line expressing active hCAII showed more rapid initial re-alkalinization of cytoplasm after induced CO2 acid load. Results also suggest that cellular pHi fine tuning was performed by the Cl-/HCO3- exchanger (AE) and Na-dependent Cl-/HCO3- exchanger (NCBE) instead of the Na+/H+ exchanger (NHE1).
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